Lady, Play Your Mandolin! is the first Merrie Melodies cartoon, released in 1931. This short has rarely been shown since its original release. It was shown as part of the Toon Heads special The Lost Warner Bros. Cartoons.
Animal patrons at a Mexican cafe sing the title song. Foxy rides up on his horse but when the horse tries to go inside, he ties its neck around a cactus. He enters the cafe and sings. Roxy takes the stage playing her mandolin and Foxy joins her in a duet. When the horse frees itself and sings with them, Foxy steps out and clobbers it with a bottle. Foxy and Roxy finish their song but as the music plays out,
the horse stumbles in, drinks a bottle of firewater, begins hallucinating, and dances so fast that he catches fire. A mouse climbs out of the horse's singed pants and slams a garbage can lid for the final cymbal clang.
This short marks the first appearance of Foxy and Roxy.
Public domain in 1960 due to Sunset Productions/Guild Films, owner of the shorts, failing to renew the copyright in time.
This cartoon was an anomaly of the selling of copyright to Sunset and Associated Artists Productions. The 1931-33 Merrie Melodies were sold to a.a.p., but this cartoon was sold to Sunset, along with the black and white 1933-34 Merrie Melodies. It is currently unknown why this happened. As a result, the short does not have a Turner "dubbed version", like all of the other 1931-33 Merrie Melodies do, sans "Hittin' the Trail for Hallelujah Land".